Detection of irradiated wheat using the electron paramagnetic resonance spin probe technique
D Dadayli, MM Sunnetcioglu, H Koksel, S Çelik
article (Uluslararası, Hakemli) | Yazar(lar): D Dadayli, MM Sunnetcioglu, H Koksel, S Çelik | Yıl: 1997 | Dergi/Kurum: CEREAL CHEMISTRY | İndeks: WOS.SCI — Özet: In this study, the electron paramagnetic resonance (EPR) spin probe technique is applied as a detection method in the differentiation of irradiated and unirra
In this study, the electron paramagnetic resonance (EPR) spin probe technique is applied as a detection method in the differentiation of irradiated and unirradiated wheat seeds. Two wheat cultivars, Kunduru and Bezostaya, were used. Aqueous solutions of 4-hydroxy-TEMPO (TANOL) spin probe prepared with a line-broadening agent, potassium ferricyanide, were used in all experiments. The EPR spectra of the samplea were recorded against time. A decrease in the signal intensity and a change in the shape of the intensity-time curve (rehydration curve) were observed, depending on the applied level of irradiation. The ratio of the lipid and aqueous regions at the high field (m(I) = -1) line changes, depending on the dose of irradiation.