Evaluation of oxidative stress cycle in healthy and inflamed dental pulp tissue: a laboratory investigation
H. Dogan Buzoglu, Mehmet Ozcan, O. Bozdemir, K. S. Aydin Akkurt, N. D. Zeybek, Y. Bayazit
article (Uluslararası, Hakemli) | Yazar(lar): H. Dogan Buzoglu, Mehmet Ozcan, O. Bozdemir, K. S. Aydin Akkurt, N. D. Zeybek, Y. Bayazit | Yıl: 2023 | Dergi/Kurum: CLINICAL ORAL INVESTIGATIONS | İndeks: WOS.SCI | DOI: 10.1007/s00784-023-05203-y | Anahtar Kelimeler: Dental pulp, GSH, GST, Irreversible pulpitis, Oxidative
Objectives The purpose of this study was to investigate the oxidative stress cycle consisting of reactive oxygen molecules (ROS), glutathione (GSH) and glutathione S-transferase (GST) in caries-related pulp inflammation.Methodology Fifty-four pulp tissue samples were collected from healthy donors with the diagnosis of reversible pulpitis, symptomatic irreversible pulpitis, and healthy pulp. Twelve pulp samples from each group were homogenized and total protein, ROS, GSH, and GST were measured by spectrophotometer. The remaining 6 samples from each group were prepared for paraffin block and used for the histopathologic and immunohistochemical evaluation of oxidative stress parameters and TUNEL labeling. Data were analyzed statistically.Results The results revealed that total protein levels significantly decreased; however, ROS levels increased in both reversible and irreversible pulpitis compared to the healthy pulp (p < 0.01). Also, as inflammation increases, GST enzyme levels decrease while GSH levels increase significantly (p < 0.05). It was found that the number of TUNEL (+) cells was increased in irreversible pulpitis samples compared to healthy and reversible pulpitis groups (p < 0.05). GSTP1 and GSH immunoreactivity were also observed in irreversible pulpitis samples.Conclusions It has been revealed that caries-related inflammation alters the oxidative stress cycle in dental pulp tissue. The increase in GSH levels in the inflamed dental pulp due to the increase in ROS levels may improve the defensive ability of the dental pulp.