Collagen-infilled 3D printed scaffolds loaded with miR-148b-transfected bone marrow stem cells improve calvarial bone regeneration in rats
Kazim K. Moncal, Dong N. Heo, Scott M. Tucker, Gregory S. Lewis, Ibrahim T. Ozbolat, R. Seda Tigli Aydin, Daniel Hayes, Mohammad Abu-Laban, Elias Rizk
article (Uluslararası, Hakemli) | Yazar(lar): Kazim K. Moncal, Dong N. Heo, Scott M. Tucker, Gregory S. Lewis, Ibrahim T. Ozbolat, R. Seda Tigli Aydin, Daniel Hayes, Mohammad Abu-Laban, Elias Rizk | Yıl: 2019 | Dergi/Kurum: MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | İndeks: WOS.SCI | DO
Differentiation of progenitors in a controlled environment improves the repair of critical-sized calvarial bone defects; however, integrating micro RNA (miRNA) therapy with 3D printed scaffolds still remains a challenge for craniofacial reconstruction. In this study, we aimed to engineer three-dimensional (3D) printed hybrid scaffolds as a new ex situ miR-148b expressing delivery system for osteogenic induction of rat bone marrow stem cells (rBMSCs) in vitro, and also in vivo in critical-sized rat calvarial defects. miR-148b-transfected rBMSCs underwent early differentiation in collagen-infilled 3D printed hybrid scaffolds, expressing significant levels of osteogenic markers compared to non-transfected rBMSCs, as confirmed by gene expression and immunohistochemical staining. Furthermore, after eight weeks of implantation, micro-computed tomography, histology and immunohistochemical staining results indicated that scaffolds loaded with miR-148b-transfected rBMSCs improved bone regeneration considerably compared to the scaffolds loaded with non-transfected rBMSCs and facilitated near-complete repair of critical-sized calvarial defects. In conclusion, our results demonstrate that collagen-infilled 3D printed scaffolds serve as an effective system for miRNA transfected progenitor cells, which has a promising potential for stimulating osteogenesis and calvarial bone repair.