The effects of bortezomib on the ovariectomy applied rat uterus: A histopathological, stereological, and immunohistochemical study
Habib Khoshvaghti, Berrin Zuhal Altunkaynak
article (Uluslararası, Hakemli) | Yazar(lar): Habib Khoshvaghti, Berrin Zuhal Altunkaynak | Yıl: 2018 | Dergi/Kurum: IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES | İndeks: WOS.SCI | DOI: 10.22038/ijbms.2018.24756.6152 | Anahtar Kelimeler: Bortezomib, Microscopy, Ovariectomy, Rat, Stereology, Uterus — Özet: Objective(s): I
Objective(s): In this study, potential protective effects of Bortezomib (Bort), as a proteasome inhibitor, were investigated on the uterus of ovariectomized rats by histological, morphometric and immunohistochemical methods.
Materials and Methods: In this study, 18 Sprague dawley strain female rats (12 weeks old, 250-300 g body weight) were used. Animals in the control group (Cont, n=6) were not exposed to any treatment. Ovariectomy was performed on the experimental groups. They (n=12) were divided into ovariectomy (Ovt, n=6) and Bortezomib (Bort, n=6) subgroups. Twelve weeks later, the rats were perfused. Then, uterine tissues were removed and examined by morphometrical, and light and electron microscopy methods. In addition, immunoreactivity of nuclear factor-kappa (NF-kappa B) was evaluated.
Results: Morphometric and histopathological evaluations showed that Bort was effective in the uterus and protects the layer structures and the cells.
Conclusion: In the light of these findings, we suggest that for proteasome inhibitor particularly Bort is thought to be useful through proteasome inhibition and NF-kappa B pathway.