Effect of Oophorectomy and Exogenous Estrogen Replacement on Liver Injury in Experimental Obstructive Jaundice
Hamdi Bulent Ucan, Mehmet Kaplan, B. Bulent Mentes, Bulent Salman, Sadrettin Pence, Cemalettin Aybay, Gulen Akyol
article (Uluslararası, Hakemli) | Yazar(lar): Hamdi Bulent Ucan, Mehmet Kaplan, B. Bulent Mentes, Bulent Salman, Sadrettin Pence, Cemalettin Aybay, Gulen Akyol | Yıl: 2007 | Dergi/Kurum: EUROPEAN JOURNAL OF THERAPEUTICS | İndeks: WOS.ESCI | DOI: 10.58600/eurjther.2007-13-2-1373-arch | Anahtar Kelimeler: Estrogen, Liver
The aim of this study was to investigate the role of estrogen on liver injury in an experimental obstructive jaundice (bile duct obstruction) model, commonly faced with in the surgical practice. Three groups of female rats were constituted; group 1 was oophorectomized and given E2 (n=14), group 2 was oophorectomized and given placebo (n=14), and group 3 was sham operated to keep blood estrogen level in the normal. range (n=14). In all groups, 14 days following constitution of bile duct ligation, serum tests and the histopathologic parameters were assessed and tissue levels of IFN-gamma and IL-6 were measured by ELISA method. Then all groups were compared. Sertun GGT and ALT levels were significantly higher in group 1 and 3 than group 2 (p<0.05). It is found that, the parameters representing both the injruy and/or the reactive response and healing were more pronounced in groups 1 and 2 (p<0.05). In the sham operated or E2 given groups significantly lower tissue levels of IFN-gamma and higher IL-6 levels were found, contrary to this, high LFN-gamma and low IL-6 tissue levels were found in the oophorectomized and placebo given group (p<0.001). The Kupffer cell alterations were observed to he more pronounced in the groups 1 and 3 (p<0.05). Our study indicates that E2 impaired the liver Sanctions, accelerated both the liver damage and healing. In the conditions of bile duct obstruction, estrogen significantly changed the cytokine milieu in the liver and also might change liver injury through the Kupffer cells that could modify the cytokine milieu.