Fecal carriage of extended-spectrum beta-lactamase-producing <i>Escherichia coli</i> and <i>Klebsiella</i> spp. in a Turkish community
Derya Cakir Erdogan, Fusun Comert, Canan Kulah, Elif Aktas, F. Kokturk
article (Uluslararası, Hakemli) | Yazar(lar): Derya Cakir Erdogan, Fusun Comert, Canan Kulah, Elif Aktas, F. Kokturk | Yıl: 2017 | Dergi/Kurum: TURKISH JOURNAL OF MEDICAL SCIENCES | İndeks: WOS.SCI | DOI: 10.3906/sag-1512-9 | Anahtar Kelimeler: Extended-spectrum beta-lactamase, Escherichia coli, Klebsiella spp, fecal c
Background/aim: The aim of this study was to determine the prevalence of fecal carriage of extended-spectrum beta-lactamase (ESBL)producing bacteria, enzyme types, and risk factors affecting colonization.
Materials and methods: A total of 576 stool samples from outpatients were examined between October 2012 and May 2013. Screening was done with selective EMB plates. ESBL were detected by double-disk synergy and confirmed agar strip gradient methods. Enzyme types were determined by PCR.
Results: The prevalence of fecal carriage was found as 30% (173 of 576). Recent use of antibiotics, hospitalization and surgical operation, diabetes, crowded household populations, and old age were associated with higher carriage rates. Of the ESBL-producing bacteria, 87.5% were positive for bla(CTX-M) genes. Of the bla(CTX-M) gene-positive isolates, 95.2% were positive for bla(CTX- M-1) genes; among these, 82.2% were positive for bla(CTX- M-3) and 67.7% were positive for bla(CTX- M-15) genes while 62.5% isolates were positive for both bla(CTX- M-3) and bla(CTX- M- 15) genes
Conclusions: A high rate (30%) of fecal carriage of ESBL bacteria was found in an adult population. The predominant beta-lactamase enzyme types were CTX- M- 3 and CTX- M- 15.