Evaluation of the effects of platelet-rich fibrin on bone regeneration in diabetic rabbits
Cenk Durmuslar, A. Ferhat Misir, Umut Balli, Figen Ongoz Dede, S. Bozkurt, Emre Baris, Zehra Yilmaz, Hakan Hamdi Celik, Alper Vatansever
article (Uluslararası, Hakemli) | Yazar(lar): Cenk Durmuslar, A. Ferhat Misir, Umut Balli, Figen Ongoz Dede, S. Bozkurt, Emre Baris, Zehra Yilmaz, Hakan Hamdi Celik, Alper Vatansever | Yıl: 2016 | Dergi/Kurum: JOURNAL OF CRANIO-MAXILLOFACIAL SURGERY | İndeks: WOS.SCI | DOI: 10.1016/j.jcms.2015.11.009 | Anahtar Kelimele
Objectives: This study aimed to investigate the effect of platelet-rich fibrin on bone regeneration in critical size defects in the calvaria of diabetic rabbits.
Study design: In total, 40 male New Zealand rabbits, were divided into two groups a non-diabetic control group (Group A) and a diabetic experimental group (Group B). Two bicortical circular defects 15 mm in diameter were created in the parietal bone of each animal. Each group was further divided into four
groups: subgroup E, the defect was left empty; subgroup PRF, the defects were filled only with PRF; subgroup AB, the defects were filled with autogenous bone; subgroup AB + PRF, the defects were filled with autogenous bone combined with PRF. The animals sacrificed at 4 weeks and 8 weeks. Bone formation was assessed by micro-computed tomography (micro-CT) scanning, histological and histo-morphometric analysis.
Result: The total percent of new bone was the lowest in group A-E (6.77 +/- 0.21 at 4 weeks, 11.01 +/- 0.37 at 8 weeks) and highest in group A-AB + PRF (21.66 +/- 0.91 at 4 weeks, 37.46 +/- 1.25 at 8 weeks; p < 0.05). The mean percent of new bone was greatest in group B-AB + PRF at 4 and 8 weeks (16.87 +/- 0.92, 29.59 +/- 1.09, respectively) and lowest in group B-E (5.83 +/- 0.09 at 4 weeks, 7.36 +/- 1.02 at 8 weeks).
Conclusion: This study, despite its limitations, showed that PRF can be used safely and that PRF induced bone healing in diabetic rabbits. (C) 2015 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.