Rectal dexmedetomidine in rats: evaluation of sedative and mucosal effects
Volkan Hanci, S. Yurtlu, K Gulle, Meryem Akpolat, Fatma Zehra Yuce, K. Karakaya, Mehmet Fatih Yuce, None Özkoçak
article (Uluslararası, Hakemli) | Yazar(lar): Volkan Hanci, S. Yurtlu, K Gulle, Meryem Akpolat, Fatma Zehra Yuce, K. Karakaya, Mehmet Fatih Yuce, None Özkoçak | Yıl: 2015 | Dergi/Kurum: REVISTA BRASILEIRA DE ANESTESIOLOGIA | İndeks: WOS.SCI | DOI: 10.1016/j.bjane.2013.09.005 | Anahtar Kelimeler: Dexmedetomidine, Rectum
Background and objectives: In this study, we investigated the anesthetic and mucosal effects of the rectal application of dexmedetomidine to rats.
Methods: Male Wistar albino rats weighing 250-300 g were divided into four groups: Group S (n=8) was a sham group that served as a baseline for the normal basal values; Group C (n=8) consisted of rats that received the rectal application of saline alone; Group IPDex (n=8) included rats that received the intraperitoneal application of dexmedetomidine (100 mu g kg(-1)); and Group RecDex (n=8) included rats that received the rectal application of dexmedetomidine (100 mu g kg(-1)). For the rectal drug administration, we used 22 G intravenous cannulas with the stylets removed. We administered the drugs by advancing the cannula 1 cm into the rectum, and the rectal administration volume was 1 mL for all the rats. The latency and anesthesia time (min) were measured. Two hours after rectal administration, 75 mg kg(-1) ketamine was administered for intraperitoneal anesthesia in all the groups, followed by the removal of the rats' rectums to a distal distance of 3 cm via an abdominoperineal surgical procedure. We histopathologically examined and scored the rectums.
Results: Anesthesia was achieved in all the rats in the Group RecDex following the administration of dexmedetomidine. The onset of anesthesia in the Group RecDex was significantly later and of a shorter duration than in the Group IPDEx (p<0.05). In the Group RecDex, the administration of dexmedetomidine induced mild-moderate losses of mucosal architecture in the colon and rectum, 2h after rectal inoculation.
Conclusion: Although 100 mu g kg(-1) dexnnedetomidine administered rectally to rats achieved a significantly longer duration of anesthesia compared with the rectal administration of saline, our histopathological evaluations showed that the rectal administration of 100 mu g kg(-1) dexmedetomidine led to mild-moderate damage to the mucosal structure of the rectum. (C) 2013 Sociedade Brasileira de Anestesiologia. Published by Elsevier Editora Ltda. All rights reserved.