POTATO LEAF EXTRACT AND ITS COMPONENT, α-SOLANINE, EXERT SIMILAR IMPACTS ON DEVELOPMENT AND OXIDATIVE STRESS IN <i>Galleria mellonella</i> L.
Zbigniew Adamski, Pawel Marciniak, Kazimierz Ziemnicki, Ender Buyukguzel, Meltem Erdem-Kucuk, K Büyükgüzel, Emanuela Ventrella, Patrizia Falabella, Massimo Cristallo, Rosanna Salvia, Sabino Aurelio Bufo, Laura Scrano
article (Uluslararası, Hakemli) | Yazar(lar): Zbigniew Adamski, Pawel Marciniak, Kazimierz Ziemnicki, Ender Buyukguzel, Meltem Erdem-Kucuk, K Büyükgüzel, Emanuela Ventrella, Patrizia Falabella, Massimo Cristallo, Rosanna Salvia, Sabino Aurelio Bufo, Laura Scrano | Yıl: 2014 | Dergi/Kurum: ARCHIVES OF INSECT BIOCHEMISTR
Plants synthesize a broad range of secondary metabolites that act as natural defenses against plant pathogens and herbivores. Among these, potato plants produce glycoalkaloids (GAs). In this study, we analyzed the effects of the dried extract of fresh potato leaves (EPL) on the biological parameters of the lepidopteran, Galleria mellonella (L.) and compared its activity to one of the main EPL components, the GA alpha-solanine. Wax moth larvae were reared from first instar on a diet supplemented with three concentrations of EPL or alpha-solanine. Both EPL and alpha-solanine affected survivorship, fecundity, and fertility of G. mellonella to approximately the same extent. We evaluated the effect of EPL and alpha-solanine on oxidative stress in midgut and fat body by measuring malondialdehyde (MDA) and protein carbonyl (PCO) contents, both biomarkers of oxidative damage. We evaluated glutathione S-transferase (GST) activity, a detoxifying enzyme acting in prevention of oxidative damage. EPL and alpha-solanine altered MDA and PCO concentrations and GST activity in fat body and midgut. We infer that the influence of EPL on G. mellonella is not enhanced by synergistic effects of the totality of potato leaf components compared to alpha-solanine alone. (C) 2014 Wiley Periodicals, Inc.