Evaluation of hepatitis C virus RNA stability in room temperature and multiple freeze-thaw cycles by COBAS AmpliPrep/COBAS TaqMan HCV
Fusun Comert, Elif Aktas, H. Agah Terzi, Canan Kulah, Y Ustundag, Selim Aydemir, F. Kokturk
article (Uluslararası, Hakemli) | Yazar(lar): Fusun Comert, Elif Aktas, H. Agah Terzi, Canan Kulah, Y Ustundag, Selim Aydemir, F. Kokturk | Yıl: 2013 | Dergi/Kurum: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | İndeks: WOS.SCI | DOI: 10.1016/j.diagmicrobio.2012.09.017 | Anahtar Kelimeler: HCV RNA stability, COBAS Am
assess the stability of various sample types and storage conditions for quantitative detectability of hepatitis C virus (HCV) RNA viral loads, we studied serum and EDTA/citrate plasma samples obtained from 10 patients known to be positive for HCV RNA. Samples were subjected to the following conditions: 1) 10 freeze-thaw (FT) cycles, and 2) storage at room temperature for 24, 48, and 72 h. Detection of HCV RNA was performed by COBAS AmpliPrep/COBAS TaqMan HCV. The following conclusions were reached: I) no significantly different viral loads were observed in different blood compartments; 2) no significantly different viral loads were observed after 24, 48, and 72 h at room temperature; 3) no significantly different viral loads were observed after 10 FT cycles in serum and plasma samples; and 4) HCV RNA is quite stable in serum and plasma (EDTA/citrate) samples. (C) 2013 Elsevier Inc. All rights reserved.