Can Lymphatic Vascular Density Be Used in Determining Metastatic Spreading Potential of Tumor in Invasive Ductal Carcinomas?
Nilufer Onak Kandemir, F Barut, Sibel Bektas, Sukru Oguz Ozdamar
article (Uluslararası, Hakemli) | Yazar(lar): Nilufer Onak Kandemir, F Barut, Sibel Bektas, Sukru Oguz Ozdamar | Yıl: 2012 | Dergi/Kurum: PATHOLOGY & ONCOLOGY RESEARCH | İndeks: WOS.SCI | DOI: 10.1007/s12253-011-9436-1 | Anahtar Kelimeler: Breast carcinoma, D2-40, Lymhangiogenesis, Lymphatic vessel invasion — Özet: Reg
Regional lymph node status is the primary parameter determining treatment strategies and prognoses in breast cancer. Lymphatic vessels in primary tumor tissue play a significant role in lymphatic metastasis. The aim of this study was to investigate the correlation of intra- and peritumoral lymphatic microvessel densities (LVD) with prognostic parameters in breast cancer, including lymphatic invasion (LI). Lymphangiogenesis was investigated using D2-40 monoclonal antibody in 69 invasive ductal carcinoma cases who underwent mastectomy and axillary lymph node dissection. Positively stained microvessels were counted at 400x in dense lymphatic vascular foci (hot-spots). Tumor LI was established when at least one neoplastic cell cluster was clearly visible inside a D2-40-positive lymph vessel. Relationships were sought between clinicopathological parameters and mean LVD and LI in primary tumor tissue. Peritumoral LVD was markedly higher than intratumoral LVD (p<0.001). No significant relationship was found between intratumoral LVD and clinicopathological parameters (p>0.05). However, significant relationships were detected between peritumoral LVD and LVI [H&E] (p=0.04), number of lymphatic invasion [n/mm2, D2-40] (p=0.001), tumor size (p=0.01), lymph node status (p=0.03), and tumor stage (p=0.04). The immunohistochemical determination of LI and LVD can contribute to the prediction of a tumor's biological behavior in invasive ductal carcinomas. Peritumoral LVD in primary tumor tissue is closely related to parameters influencing the prognosis of a tumor.