Role of angiotensin and endothelin in testicular ischemia reperfusion injury
Zehra Kurcer, Gunnur Ozbakis Dengiz, ZN Banoglu, Nilufer Onak Kandemir, A. Gorkem Mungan, VH Ozacmak, Burak Turkili
article (Uluslararası, Hakemli) | Yazar(lar): Zehra Kurcer, Gunnur Ozbakis Dengiz, ZN Banoglu, Nilufer Onak Kandemir, A. Gorkem Mungan, VH Ozacmak, Burak Turkili | Yıl: 2012 | Dergi/Kurum: INTERNATIONAL JOURNAL OF UROLOGY | İndeks: WOS.SCI | DOI: 10.1111/j.1442-2042.2011.02924.x | Anahtar Kelimeler: Bosentan, enalapril
Objectives: To determine whether angiotensin and endothelin have any role in testicular ischemia reperfusion injury by investigating the effects of the angiotensin converting enzyme inhibitor enalapril, selective non-peptide angiotensin-II type I blocker losartan and dual endothelin receptor blocker bosentan.
Methods: Rats were anesthetized with thiopental sodium (50 mg/kg i.p.) before the operation. The left testicular artery and vein of rats were occluded for 1 h; before the bilateral orchiectomy, the organ was allowed to reperfuse for 3 h or 24 h. Enalapril (20 mg/kg i.p.), losartan (30 mg/kg i.p.), bosentan (10 mg/kg i.p.) or vehicle (saline) were given 30 min before reperfusion. Malondialdehyde level was measured in testicular tissue after 3 h of reperfusion. Histological examination was carried out after 24 h of reperfusion.
Results: Ischemia reperfusion caused a significant increase in malondialdehyde level of ipsilateral testis, and histopathological injury in both ipsilateral and contralateral testes. Enalapril, losartan and bosentan treatments prevented the ischemia reperfusion-induced augmentation in malondialdehyde levels. Only bosentan treatment ameloriated ischemia reperfusion-induced histopathological alterations.
Conclusions: Endothelin might play a more important role in pathogenesis of testicular ischemia reperfusion injury when compared with angiotensin.