Determination of low level nitrite and nitrate in biological, food and environmental samples by gas chromatography-mass spectrometry and liquid chromatography with fluorescence detection
Mehmet Akyuz, Sevket Ata
article (Uluslararası, Hakemli) | Yazar(lar): Mehmet Akyuz, Sevket Ata | Yıl: 2009 | Dergi/Kurum: TALANTA | İndeks: WOS.SCI | DOI: 10.1016/j.talanta.2009.05.016 | Anahtar Kelimeler: Nitrite and nitrate, NAT, GC-MS and LC-FL, Biological, Food and environmental samples — Özet: Gas chromatography-mass spectrometry (GC-MS)
Gas chromatography-mass spectrometry (GC-MS) and liquid chromatography with fluorescence detection (LC-FL) methods have been proposed for the determination of low level nitrite and nitrate in biological, food and environmental samples. The methods include derivatization of aqueous nitrite with 2.3-diaminonaphthalene (DAN), enzymatic reduction of nitrate to nitrite, extraction with toluene and chromatographic analyses of highly fluorescent 2,3-naphthotriazole (NAT) derivative of nitrite by using GC-MS in selected-ion-monitoring (SIM) mode and LC-FL Nitrite and nitrate ions in solid samples were extracted with 0.5 M aqueous NaOH by sonication. The recoveries of nitrite and nitrate ions based on CC-MS and LC-FL results were 98.40% and 98.10% and the precision of these methods, as indicated by the relative standard deviations (RSDs) were 1.00% for nitrite and 1.20% for nitrate, respectively. The limits of detection of the GC-MS in SIM mode and LC-FL methods based on S/N = 3 were 0.02 and 0.29 pg/ml for nitrite and 0.03 and 0.30 pg/ml for nitrate, respectively. (C) 2009 Elsevier B.V. All rights reserved.