Protective effects of L-arginine on rat terminal ileum subjected to ischemia/reperfusion
H Sayan, VH Ozacmak, RG Aktas, SO Arslan, S Altaner
article (Uluslararası, Hakemli) | Yazar(lar): H Sayan, VH Ozacmak, RG Aktas, SO Arslan, S Altaner | Yıl: 2008 | Dergi/Kurum: JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION | İndeks: WOS.SCI | Anahtar Kelimeler: contractile activity, ileum, ischemia and reperfusion, nitric oxide — Özet: Objectives: Studies have sho
Objectives: Studies have shown that nitric oxide (NO) may play a major role in sustaining mucosal integrity; however, NO has been also implicated in the pathogenesis of ischemia/ reperfusion (I/R)-related tissue injury. We investigated the effects of L-arginine and N-G-nitro L-arginine methyl ester (L-NAME) on the acetylcholine-induced contractile response of ileum and the levels of malondialdehyde (MDA) and reduced glutathione (GSH). Histopathological changes were also evaluated in ileal preparations.
Materials and Methods: Mate Wistar Albino rats were subjected to mesenteric ischemia (30 min) followed by reperfusion (3 hours). Four groups were designed: sham-operated control; I/R; I/R and L-arginine pretreatment; and I/ R and L-NAME pretreatment. After reperfusion, ileum specimens were collected to determine the parameters mentioned above.
Results: Following reperfusion, a significant decrease in acetylcholine-induced contractile response, an increase in lipid peroxidation, a decrease in GSH content, and mucosal damage of the ileal preparations were observed. We showed that decreased contractility, increased lipid peroxidation, and reduced GSH content have been reversed by L-arginine but not by L-NAME. Mucosal injury was significantly lowered in the L-arginine group.
Conclusions: Treatment with L-arginine exerted a protective effect in intestinal I/R injury, which was mediated in part by regulating MDA and GSH levels, consequently ameliorating impaired contractile response and mucosal injury.